Coding

Part:BBa_K1175007:Design

Designed by: Nicholas K. Goldner   Group: iGEM13_WLC-Milwaukee   (2013-09-17)


beta-galacturonidase (YesZ) from Bacillus Subtilis 168


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 260
    Illegal NgoMIV site found at 395
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 559


Design Notes

No design considerations were needed, although we did add a second stop codon.


Source

Bacillus Subtilis 168 genomic DNA

References